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2010.01.20 - Kyle Allen - Joint pain with small animal models

Evaluating pain sensitivity and joint dysfunction in small animal models in musculoskeletal disease - Kyle Allen

  • Works with Dr. Setton at Duke has a K99 <-check out when you are a post-doc
  • Joint disease
    • Loss of proteoglycans
    • Surface fibrillation
    • Affects entire joint not just cartilage
  • Define pain related behaviors in rodents
    • Developing a phenotype that actually correlates with disease
    • Type IX collagen knock-out
      • Causes OA-like lesions
      • Have similar geometry in skeleton to wild-type
    • Test force at which the animals will withdraw from shows a difference between KO and WT
    • Measured gait parameters in KO
      • Stride length shorter
      • Contact on the ground longer
  • Found that the best predictor of knee OA in rodent model with Collagen IX knock-out
    • Force sensitivity is probably one of the best predictors of OA
  • Problems with this model are that OA is usually a local pathologies not a global one.
  • Interleukin-1B up-regulation seems to correspond to OA and addition of IL1 has been shown to improve cartilage.
  • Gait perturbations when messing with dorsal root ganglion, but returns back to normal gait kinematics.
  • Injection of TNF appears to reduce symptoms of lower back pain.
  • Want to use ground reaction forces as a predictor of musculoskeletal diseases
  • Medial lateral forces are reduced in rats with lower back pain