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09.15.2009: Mellisa Kemp - Redox Regulation of Signaling Proteins

ROS - reactive oxygen species
  • Lots of different cell types produce ROS
    • Changes binding affinity of proteins
  • Is hydrogen-peroxide a signaling mechanism?
  • Use cue->signal->response method (treat cell as a black box).
  • SSG can inhibit phosphorolation
  • Thiol modifications are controlled by the redox environment.
  • By understanding physiological case we can understand pathological cases.
  • Creation of ODE model, fit parameters to measurements, predicted chemical levels and provided insight to which pathways were most important.
  • Can ROS be used to change the "gain" of pathways in a cell?
  • ROS receptors are primarily membrane bound.
  • Microfluidics are used for cell fixing, lysing, etc.
    • Utilization of cell traps using microfluidics.  Can this be used in MEAs?
  • Real-time imaging of ROS pathways is accomplished using genetically inserted fluorophors.
  • Thyrodoxin appears to be distributed primarily in the ER.
Questions
  • Thyrodoxin, does it move from the lysosomes to the endosomes? Unknown.
  • Thyrodoxin, where is it folded?  Unknown, thyrodoxin is a small protein and doesn't need much folding.
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